Growth and differentiation of neural stem cells from neurospheres after culture in serum-free medium

doi:10.3969/j.issn.2095-4344.2013.14.018

Abstract

Abstract:

BACKGROUND: In vitro isolation and culture of neural stem cells with high purity, strong viability and homogeneous biological characteristics are the basis of neural stem cell transplantation.
OBJECTIVE: To establish a method of isolation, cultivation and purification of rat neural stem cells in vitro, to observe cell morphology, and to assess surface markers and multi-directional differentiation capacity.
METHODS: Neural stem cells were isolated from rat embryonic cerebral cortex and cultured in serum-free medium. Immunocytochemical staining was used to examine the markers of neural stem cells and their differentiated cells. Cell surface markers were assessed by flow cytometry.
RESULTS AND CONCLUSION: Neural stem cells grew as free-floating neurospheres in the serum-free conditions.This method is simple and can isolate, purify and amplify neural stem cells in vitro. The obtained cells have the general biological characteristics of neural stem cells, accounting for 91.5% by flow cytometry at passage 3, and also have multi-directional differentiation potential. Immunocytochemical staining and flow cytometry demonstrated that neural stem cells can differentiate into neurons, astrocytes and oligodendrocytes.

Key words: stem cells, stem cell culture and differentiation, neural stem cells, neurospheres, cell culture, serum-free, suspension, flow cytometry, stem cell photographs-containing paper

CLC Number:

R394.2

Zhao Ji-tong, Shen Qiang, Huang Fei. Growth and differentiation of neural stem cells from neurospheres after culture in serum-free medium[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(14): 2592-2596.

Figures/Tables 5

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